Transposon Technology:
from research to applications

Efficient Gene Transfer to Cells

Efficient Transgenesis

research
and applications

Applicable to basic research, gene therapy, Car-T therapy, protein production, antibody drug production etc.

More than
10 times!

The transposon technology increases efficiencies of gene transfer to many cell types including iPS cells, ES cells, primary culture cells etc.

More than
10 times!

The transposon technology increases efficiencies to construct transgenic animals. Any vertebrate animals used for research to agricultural purposes.

The reason why the transposon technology is so successful

The transposon technology has been licensed to more than 20 major Japanese and international drug companies!

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Easy handling!

The transposon can be handled by a standard method for DNA manipulation. It is neither hazardous nor laborious compared to viral vectors.

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Highly efficient gene transfer！

The transposon technology increases efficiencies of gene transfer to iPS cells, ES cells, primary culture cells, etc. and trans genesis of vertebrate animals, from fish to mammals.

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Efficient protein production！

Cells and animals created by the transposon technology produce gene products (proteins) encoded by the transgene very efficiently.

The transposon technology saves cost for gene transfer to cells and animals

 
トランスポゾン

Transposon is a mobile genetic element

Transposon jumps from one locus to another via the transposase activity. This feature is used for gene transfer.

実験

Cloning into a transposon vector

Clonig a gene of interest into a transposon vector. This step can be done by standard DNA manipulations.

Integration through transposition

The transposon vector is integrated into the genome through transposition very efficiently.

   

   

Successful examples from companies that used the transposon technology

Japanese drug company K

Success in production of antibody using CHO cells.

Japanese drug company D

Success in gene transfer to iPS cells. Established many useful cell lines.

Japanese drug company C

Success in making transgenic mice. Established many useful transgenic mouse lines.

US drug company K

Success in making transgenic zebrafish.

国立遺伝学研究所川上浩一教授Prof. Koichi Kawakami, who developed the transposon technology

Laboratory of Molecular and Developmental Biology

National Institute of Genetics

Received Ph D from University of Tokyo. Working at Massachusetts Institute of Technology (1994~1997) and started the zebrafish research. After coming back to Japan, developed the transposon technology and established a method to construct transgenic zebrafish and mice. Published more than 200 papers. Founding board members of International Zebrafish Society (2014~) and Zebrafish Disease Models Society (2014~).

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